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- KITAGAWA YOSHICHIKA
- Department of Biochemistry, Akita University School of Medicine
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- MATSUMOTO TSUTOMU
- Department of Biochemistry, Akita University School of Medicine Akita Agricultural College
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- OKUHARA EIJI
- Department of Biochemistry, Akita University School of Medicine
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- SHIKATA EISHIRO
- Department of Botany, Faculty of Agriculture, Hokkaido University
書誌事項
- タイトル別名
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- Immunogenicity of Rice Dwarf Virus Ribo
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抄録
Using rice dwarf virus (RDV)-RNA which was extracted from RDV and further purified by MAK-column chromatography, anti-RDV-RNA antibodies were produced in rabbits immunized with RDV-RNA-methylated bovine serum albumin complexes. The antisera, as analzyed by complement fixation, cross-reacted with synthetic double stranded RNAs (poly (A)•poly (U), poly (I)•poly (C)), but not with native or denatured DNA, rRNA, tRNA, 5 S RNA and nucleic acids from rice plants. RDV-RNA treated with heat or dimethylsulfoxide was markedly reduced in reactivity to the antisera. When RDV-RNA was digested with RNase A at low salt concentration, its complement fixation activity was abolished. In double diffusion tests, two different precipitation lines were demonstrated between the antiserum and RDV-RNA. One of the precipitation lines connected with those was formed between the antisera, poly (A)•poly (U) and poly (I)•poly (C). As regards immunoglobulin classes of the antibodies, one of the two rabbits employed had antibody activity only in IgM and the other, exclusively in IgG.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 122 (4), 337-343, 1977
東北ジャーナル刊行会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282679218495616
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- NII論文ID
- 130003492343
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- NII書誌ID
- AA00863920
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- COI
- 1:CAS:528:DyaE2sXls1Grsr4%3D
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- ISSN
- 13493329
- 00408727
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- NDL書誌ID
- 1912564
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- PubMed
- 918970
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可