The Shortest Peroxidase Stain Time and the Mean Cellular Peroxidase Content of Blood Leukocytes

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  • Higashi Ototaka
    Department of Pediatrics, Iwate Medical College Morioka and Department of Pediatrics (Prof. Ts. Arakawa), Faculty of Medicine, Tohoku University Department of Pediatrics, Faculty of Medicine, Tohoku University

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A new modification of the shortest peroxidase stain time (SPST) method for blood leukocytes, originally studied by Suzuki, based on the Sato-Sekiya's stain, was described. This method can be used for a quantitative determination of the peroxidase activity of leukocytes. The rationale of the method was demonstrated.<br> The mean cellular peroxidase content (MCP) of myelogenous leukocytes of ten hospitalized patients estimated by the present SPST method, was equivalent, in average, to (15.29±3.71)×10-8γ of horseradish peroxidase (HRP) in eosinophils, (8.18±4.08)×10-8γ of HRP in neutrophils and (4.97±2.72)×10-8γ of HRP in monocytes, respectively.

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