Determining the Possible Etiology of Hospital-Acquired Pneumonia Using a Clone Library Analysis in Japan
-
- Yatera Kazuhiro
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Noguchi Shingo
- Department of Respiratory Medicine, University of Occupational and Environmental Health Department of Respiratory Medicine, Wakamatsu Hospital of the University of Occupational and Environmental Health
-
- Yamasaki Kei
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Kawanami Toshinori
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Fukuda Kazumasa
- Department of Microbiology, University of Occupational and Environmental Health, Japan
-
- Naito Keisuke
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Akata Kentaro
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Kido Takashi
- Department of Respiratory Medicine, University of Occupational and Environmental Health
-
- Ishimoto Hiroshi
- Department of Respiratory Medicine, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences
-
- Sakamoto Noriho
- Department of Respiratory Medicine, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences
-
- Taniguchi Hatsumi
- Department of Microbiology, University of Occupational and Environmental Health, Japan
-
- Mukae Hiroshi
- Department of Respiratory Medicine, Unit of Translational Medicine, Nagasaki University Graduate School of Biomedical Sciences
この論文をさがす
抄録
<p>Obtaining precise etiological information regarding causative bacteria is important for the proper use of antimicrobials in hospital-acquired pneumonia (HAP), which is associated with a high rate of mortality. The aim of this study was to comparatively investigate the bacterial diversity in bronchoalveolar lavage fluid (BALF) in Japanese patients with HAP by the clone library method using the 16S rRNA gene. This study included Japanese patients with HAP who were treated at our hospital and referring hospitals. BALF specimens were obtained from pneumonia lesions identified on chest radiographs and/or computed tomography. Sputum specimens were also evaluated in patients with sputum production. Sixty-eight patients were ultimately enrolled. BALF cultivation revealed bacterial positivity in 53 of 68 (77.9%) patients, and Staphylococcus aureus (30.9%) was the most frequently isolated, followed by Pseudomonas aeruginosa (16.2%), and Escherichia coli (10.3%). In contrast, the clone library analysis identified the presence of some bacterial phenotype in 65 of 68 (95.6%) patients, and streptococci (16.2%), Corynebacterium species (11.8%), anaerobes (10.3%) were frequently detected as the predominant phylotypes. Both methods tended to detect S. aureus, Klebsiella pneumoniae, and E. coli in patients with late-onset pneumonia. In addition, the cases that phylotypes of S. aureus and P. aeruginosa were found to account for > 5% of the bacterial flora of each case were 42.9% and 72.7%, respectively. These results indicate that attention should be paid to the roles of gram-positive bacilli such as streptococci, Corynebacterium species and anaerobes, in addition to Gram-negative bacilli, in the pathogenesis of HAP.</p>
収録刊行物
-
- The Tohoku Journal of Experimental Medicine
-
The Tohoku Journal of Experimental Medicine 242 (1), 9-17, 2017
東北ジャーナル刊行会