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- Mouri Toraichi
- Department of Internal Medicine, Tohoku University School of Medicine
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説明
A practical method for the estimation of plasma kininogens I and TI was described. The level of kininogen I was calculated as difference between total kininogen and kininogen II. One-tenth milliliter of heparinized plasma was in-cubated with 1.2 Frey units of human plasma kallikrein in 0.05M phosphate buffer, pH 7.4, at 37°C for 45 minutes to deplete kininogen I completely. It was then heated at 62°C for 15 minutes and incubated with 100μg of trypsin at 37°C for 45 minutes to release bradykinin from kininogen IT. To measure the total kininogen level (kininogens I and II), 0.1ml of plasma was first heated at 62°C for 15 minutes in 0.05 M phosphate buffer, pH 7.4. It was then incubated with 100μg of trypsin at 37°C for 45 min<br>utes. Released plasma kinin was assayed on the isolated rat uterus against synthetic bradykinin as a standard. Total kininogen and kininogen II levels were expressed as the amount of bradykinin released by these procedures.<br> In 10 healthy subjects, the estimated levels for plasma kininogens I and IT lc ere 2.48±10.28 and 3.15±0.55μg bradykinin equivalent per milliliter, respeetieely. It seems essential to estimate the changes of kininogens I and II instead of total kininogen for the study of plasma kallikrein-kinin system in pathological and physiological conditions.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 98 (2), 147-153, 1969
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390282679220238848
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- NII論文ID
- 130003497979
- 40018682831
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- NII書誌ID
- AA00863920
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- COI
- 1:CAS:528:DyaE3cXjvFentQ%3D%3D
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- ISSN
- 13493329
- 00408727
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- NDL書誌ID
- 8507871
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- PubMed
- 5817913
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可