Comparison of bacterial and fungal biomass determined by phospholipid fatty acid and direct microscopical analysis in 4 types of upland soils
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- Arao Tomohito
- National Institute for Agro-Environmental Sciences
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- Okano Seigo
- Tohoku National Agricultural Experiment Station
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- Nishio Takashi
- National Agriculture Research Center
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Abstract
We investigated the relationship between the phospholipid fatty acid (PLFA) content and bacterial and fungal biomass in soils incubated in the presence/ absence of cellulose or glucose. PLFA content except for 18 : 2ω6 in soil was correlated with the bacterial biomass estimated by direct bacterial counts (r =0.75 ; p< 0.01, n=66) and the average ratio of bacterial biomass to PLFA content was 4.7±0.2 in 3 types of upland soils. These results suggest that the bacterial biomass can be estimated based on the PLFA content in soil. A positive correlation (r=0.50 ; P<0.01, n=86) was also found between the content of PLFA 18 : 2ω6 and the fungal biomass determined by direct microscopical analysis in 4 types of upland soils. This correlation coefficient increased when the soils containing PLFA 18 : 2ω6 below 0.1 mg g^<-1> dry soil were omitted (r=0.64 ; p<0.01, n=38). In this case, the average ratio of fungal biomass to PLFA 18 : 2ω6 content was 822±71. A similar average ratio was observed in a Low Humic Andosol after application of pig compost (769±149). These results suggest that the PLFA 18 : 2ω6 content could be used to estimate the fungal biomass in soils containing PLFA 18 : 2ω6 above 0.1 mg g^<-1> dry soil. The content of PLFA 18 : 2ω6 increased about three times more than did the fungal biomass just after application of pig compost. In such cases, the PLFA 18 : 2ω6 content could be a relative measure of fungal biomass.
Journal
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- Soil Microorganisms
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Soil Microorganisms 55 (1), 29-36, 2001
Japanese Society of Soil Microbiology
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Details 詳細情報について
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- CRID
- 1390282679230296064
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- NII Article ID
- 110009468067
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- NII Book ID
- AN00352796
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- ISSN
- 21896518
- 09122184
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- NDL BIB ID
- 5765357
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed