Analysis of transcriptional region found in a tomato pathotype-specific DNA fragment of Verticillium dahliae
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- Usami Toshiyuki
- Faculty of Horticulture, Chiba University
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- Amemiya Yoshimiki
- Faculty of Horticulture, Chiba University
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- Shishido Masahiro
- Faculty of Horticulture, Chiba University
Bibliographic Information
- Other Title
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- Verticillium dahilaeのトマト系菌株特異的DNA断片中に見出された転写領域の解析
- Verticillium dahliaeのトマト系菌株特異的DNA断片中に見出された転写領域の解析
- Verticillium dahliae ノ トマトケイ キンカブ トクイテキ DNA ダンペン チュウ ニ ミイダサレタ テンシャ リョウイキ ノ カイセキ
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Abstract
The DNA fragment (VDf130) specific to the tomato pathotype of Verticillium dahliae, cloned from the genomic DNA of the fungal isolate TV103, was confirmed to carry a transcriptional region. Assuming that the transcriptional region was a part of a gene (designated as vdtl), we sequenced the region containing its periphery and cDNA of the region. As a result, the transcriptional region contained a putative open reading frame coding for 137 amino acids separated by two introns. TATA box and CAAT box were also found upstream of the transcriptional region. However, vdtl or its presumed amino acid sequences did not show any significant homology to known genes or proteins in available data bases. Southern and Northern hybridization analyses showed that vdtl was found only in the isolates of the tomato pathotype among 19 tested isolates of V. dahliae and was transcribed in culture mycelia, spores, microsclerotia of TV103 as well as the tomato seedlings infected with the fungus.
Journal
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- Soil Microorganisms
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Soil Microorganisms 55 (1), 11-20, 2001
Japanese Society of Soil Microbiology
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Details 詳細情報について
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- CRID
- 1390282679230300928
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- NII Article ID
- 110009468065
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- NII Book ID
- AN00352796
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- ISSN
- 21896518
- 09122184
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- NDL BIB ID
- 5765283
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed