Fluorescence Quenching of Bovine Serum Albumin in Reversed Micelles by CdS Nanoparticles

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  • TAN Liang
    Chemical Research Institute, Hunan Normal University
  • LIU LianYing
    Center of Analyses and Tests, Hunan Normal University
  • XIE QingJi
    Chemical Research Institute, Hunan Normal University
  • ZHANG YouYu
    Chemical Research Institute, Hunan Normal University
  • YAO ShouZhuo
    Chemical Research Institute, Hunan Normal University

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Abstract

The fluorescence emission spectra and 3D fluorescence spectra of bovine serum albumin (BSA) in cetyltrimethylammonium bromide (CTAB) reversed micelles were affected by the microenvironment. Blue shifts of the fluorescence emission peaks were found when BSA was present in CTAB reversed micelles. The fluorescence intensity changed with the water content. Similar changes in the peak regions of the 3D fluorescence spectra were also observed. CdS nanoparticles prepared in CTAB reversed micelles quenched the fluorescence of BSA significantly. The fluorescence of BSA was more effectively quenched by negative CdS nanoparticles than by positive or neutral CdS ones. The quenching degree increased linearly with increasing the concentration of negative CdS nanoparticles over the range of 5.0 × 10-6 - 3.0 × 10-5 mol L-1. The quenching mechanism is discussed and the quenching constant is 1.32 × 104 L mol-1.

Journal

  • Analytical Sciences

    Analytical Sciences 20 (3), 441-444, 2004

    The Japan Society for Analytical Chemistry

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