Drug Response Assay System in a Microchip Using Human Hepatoma Cells

TANAKA Yuki, SATO Kiichi, YAMATO Masayuki, OKANO Teruo, KITAMORI Takehiko

doi:10.2116/analsci.20.411

Drug Response Assay System in a Microchip Using Human Hepatoma Cells

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TANAKA Yuki

Department of Applied Chemistry, School of Engineering, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency
SATO Kiichi

Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo Micro Chemistry Group, Special Research Laboratory for Optical Science, Kanagawa Academy of Science and Technology
YAMATO Masayuki

Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University
OKANO Teruo

Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University
KITAMORI Takehiko

Department of Applied Chemistry, School of Engineering, The University of Tokyo Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency Micro Chemistry Group, Special Research Laboratory for Optical Science, Kanagawa Academy of Science and Technology

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抄録

A microchip-based cell response assay system to an anticancer agent was developed. The hepatoma cell line HepG2 was used to assess the effects of an anticancer agent, doxorubicin. The required cell number was reduced by two orders, and the observation of the time course of cell response became possible. The system clearly showed that treatment with higher doses of the drug or longer exposure times gave more effects to cells. The possibilities of novel drug response studies or toxicity assay system were demonstrated.