Electrochemical Sensing of Glucose at a Platinum Electrode with a Chitin/Glucose Oxidase Film.

SUGAWARA Kazuharu, FUKUSHI Hiroshi, HOSHI Suwaru, AKATSUKA Kunihiko

doi:10.2116/analsci.16.1139

Electrochemical Sensing of Glucose at a Platinum Electrode with a Chitin/Glucose Oxidase Film.

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SUGAWARA Kazuharu

Department of Applied and Environmental Chemistry, Kitami Institute of Technology
FUKUSHI Hiroshi

Department of Applied and Environmental Chemistry, Kitami Institute of Technology
HOSHI Suwaru

Department of Applied and Environmental Chemistry, Kitami Institute of Technology
AKATSUKA Kunihiko

Department of Applied and Environmental Chemistry, Kitami Institute of Technology

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Chitin powder was dissolved in a mixed solution of N,N-dimethylacetoamide and 1-methyl-2-pyrrolidone with lithium chloride. The chitin solution was adhered to the film. Glucose oxidase having a negative charge was immobilized to the protonated chitin film in 0.1 mol dm^-3 acetate buffer (pH 5.0), based on electrostatic interaction. The interaction is useful and convenient for an immobilization of the enzyme. In this study, glucose sensor was developed at a platinum electrode with a chitin/glucose oxidase film as an application of chitin in analytical chemistry. An oxidation peak of H₂O₂ from the enzyme reaction was observed at +0.5 V (vs. Ag/AgCl) in 0.1 mol dm^-3 acetate buffer (pH 6.2). The calibration curve of glucose was linear in the range from 5×10^-7 to 3×10^-5 mol dm^-3. The sensor was applied to determination of glucose in sports drinks.