A Simple Preparation of Stationary Phase of Alkylamide Reversed-Phase Liquid Chromatograph for the Separation of Basic Substances.

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  • HUANG Xiaojia
    Dalian Institute of Chemical Physics, Chinese Academy of Sciences
  • WANG Junde
    Dalian Institute of Chemical Physics, Chinese Academy of Sciences
  • LIU Xueliang
    Dalian Institute of Chemical Physics, Chinese Academy of Sciences

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  • Simple Preparation of Stationary Phase of Alkylamide Reversed Phase Liquid Chromatograph for the Separation of Basic Substances

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Description

A simple preparation process of alkylamide phase for reversed-phase HPLC (RP-HPLC) is described. The process includes aminopropyltrimethoxysilane firstly reacted with octanoyl chloride, then the intermediate was coupled onto porous silica. The resultant bonded silica has a reproducible ligand surface concentration and homogenous bonded ligand distribution on the porous silica. Characterization of prepared packing was carried out with elemental analysis, solid-state 13C NMR and Fourier transform infrared (FT-IR). Chromatographic evaluations were carried out by using a mixture of organic compounds including acidic, basic and neutral analytes under methanol/water as binary mobile phase. The results showed that the stationary phase have excellent chromatographic properties and can be efficiently used for the separation of basic compounds.

Journal

  • Analytical Sciences

    Analytical Sciences 18 (1), 69-72, 2002

    The Japan Society for Analytical Chemistry

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