Headspace Solid-Phase Microextraction-Gas Chromatography Method for the Determination of Valproic Acid in Human Serum, and Formulations Using Hollow-Fiber Coated Wire

FARAJZADEH Mir Ali, FARHADI Khalil, MATIN Amir A., HASHEMI Paria, JOUYBAN Abolghasem

doi:10.2116/analsci.25.875

Headspace Solid-Phase Microextraction-Gas Chromatography Method for the Determination of Valproic Acid in Human Serum, and Formulations Using Hollow-Fiber Coated Wire

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FARAJZADEH Mir Ali

Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz
FARHADI Khalil

Department of Chemistry, Faculty of Science, Urmia University
MATIN Amir A.

Department of Chemistry, Faculty of Science, Urmia University Research Department of Chromatography, Iranian Academic Center for Education, Culture & Research (ACECR)
HASHEMI Paria

Department of Chemistry, Faculty of Science, Urmia University
JOUYBAN Abolghasem

School of Pharmacy & Drug Applied Research Center, Tabriz University of Medical Sciences

書誌事項

公開日: 2009

DOI

10.2116/analsci.25.875

公開者: 社団法人日本分析化学会

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説明

A method was developed for the extraction of valproic acid (VPA) by hollow-fiber coated wire as a lab-made solid-phase microextraction (SPME) fiber and its determination by capillary gas chromatography in human serum and pharmaceutical formulations. In this study, a piece of copper wire coated by polypropylene hollow-fiber membrane was used as a SPME fiber, and its efficiency for the extraction of VPA from the headspace of samples prior to gas chromatographic analysis was evaluated. The optimum conditions of microextraction process were selected, and the limit of detection for VPA was found to be 85 μg L⁻¹ in solution and 1.7 mg L⁻¹ in human serum. A low detection limit, a wide linear dynamic range (0.25 − 100 mg L⁻¹), good repeatability (RSD%<4 in formulations and RSD%<7 in serum samples) and a higher mechanical durability due to its metallic base are some of the most important advantages of the proposed fiber.