Enhancement of the Protein Loading Density by a Pre-Cleaning Process of a Gold Substrate: Confocal Laser Scanning Microscopic Study
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- PARK Taehan
- Department of Applied Chemistry, Hanyang University
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- CHOO Jaebum
- Department of Applied Chemistry, Hanyang University
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- LEE Moonkwon
- Micro Biochip Center, Hanyang University
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- KIM Yang S.
- Micro Biochip Center, Hanyang University
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- LEE Eun Kyu
- Micro Biochip Center, Hanyang University
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- LEE Hae Seong
- Korea Basic Science Institute
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Abstract
The immobilization of glucose oxidase (GOx), using self assembled monolayers (SAMs) on gold surfaces, was investigated by grazing angle FT-IR spectroscopy, surface plasmon resonance (SPR) spectroscopy, and atomic force microscopy (AFM) in conjunction with confocal laser scanning microscopy (CLSM). To find an optimum condition for the maximum GOx loading density on gold surfaces, different cleaning protocols were examined. The loading density of GOx on surfaces was investigated by AFM and CLSM. In particular, CLSM was more effective for identifying the GOx density than AFM, since its scanning speed is much faster and it covers a larger area. Based on CLSM images of the GOx immobilized on the surfaces, it was concluded that the pre-cleaning process of gold substrates using different solvents, such as acetone, ethanol and 2-propanol, is very important for enhancing the GOx loading density. This result enables us to investigate an effective fabrication process in fabricating biosensors.
Journal
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- Analytical Sciences
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Analytical Sciences 20 (9), 1255-1258, 2004
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679232161024
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- NII Article ID
- 130004440983
- 10013524290
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXnvVGru7o%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 7078590
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- PubMed
- 15478332
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed