Determination of 1-Hydroxypyrene in Human Urine by Acid Hydrolysis Coupled to Solid-Phase Microextraction and Semi-microcolumn Liquid Chromatography

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  • CHEN Hong-Wen
    Departmant of Environmental Engineering and Health, Yuanpei University of Science and Technology

書誌事項

公開日
2007
DOI
  • 10.2116/analsci.23.1221
公開者
社団法人 日本分析化学会

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説明

This study developed an acid hydrolysis coupled to a solid-phase microextraction method employing a semi-microcolumn liquid chromatography system, instead of enzyme hydrolysis with solid-phase extraction for the pretreatment of human urine samples, to detect urinary 1-hydroxypyrene (1-OHP). The complete separation and detection of urinary 1-hydroxyprene was performed using a high-performance liquid-chromatography fluorescence detection system with an analytical C18 semi-microcolumn, 60% (v/v) aqueous acetonitrile elution, and a λex/em = 348/388 nm pair detection wavelength. Calibration graphs were linear with very good correlation coefficients (r = 0.9997), and the detection limit was 1.0 ng/L. These important parameters for acid hydrolysis and solid-phase microextraction were investigated. The total recovery was above 83% in acid hydrolysis with solid-phase microextraction. The proposed method provided a relatively simple, convenient, and practical procedure to determine the level of urinary 1-hydroxypyrene in biological samples, and was successfully applied to detect the urine of students.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 23 (10), 1221-1225, 2007

    社団法人 日本分析化学会

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