A MutS Protein-immobilized Au Electrode for Detecting Single-base Mismatch of DNA

DOI PDF Web Site Web Site Web Site 3 Citations 33 References
  • HAN Aishan
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • TAKARADA Tohru
    Bioengineering Laboratory, RIKEN
  • SHIBATA Taiki
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • NAKAYAMA Masamichi
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • MAEDA Mizuo
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Bioengineering Laboratory, RIKEN

Bibliographic Information

Other Title
  • MutS Protein immobilized Au Electrode for Detecting Single base Mismatch of DNA

Search this article

Abstract

A novel electrochemical biosensor was developed to detect gene mutation by using a DNA-mismatch binding protein: MutS from Escherichia coli. The MutS protein was immobilized onto an Au-electrode surface via complex formation between a histidine tag of the MutS protein and a thiol-modified nitrilotriacetic acid chemically adsorbed on the Au-electrode surface. When a target double-stranded DNA having a single-base mismatch was captured by the MutS protein on the electrode, some electrostatic repulsion arose between polyanionic DNA strands and anionic redox couple ions. Consequently, their redox peak currents on a cyclic voltammogram with the Au electrode drastically decreased, depending on the concentration of the target DNA, according to the redox couple-mediated artificial ion-channel principle. By using this assay, one can detect all types of single-base mismatch and single-base deletion.

Journal

  • Analytical Sciences

    Analytical Sciences 22 (5), 663-666, 2006

    The Japan Society for Analytical Chemistry

Citations (3)*help

See more

References(33)*help

See more

Keywords

Report a problem

Back to top