Production and Characterization of Monoclonal Antibodies Recognizing the Asymmetric Center of Optically Active Bufuralol and Its Metabolites.
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- IKEGAWA Shigeo
- Graduate School of Pharmaceutical Sciences, Tohoku University
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- ISRIYANTHI Ni Made Ria
- Graduate School of Pharmaceutical Sciences, Tohoku University
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- KOBAYASHI Norihiro
- Graduate School of Pharmaceutical Sciences, Tohoku University
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- MIYAIRI Shinichi
- Graduate School of Pharmaceutical Sciences, Tohoku University
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- GOTO Junichi
- Graduate School of Pharmaceutical Sciences, Tohoku University
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A specific antibody has been produced for developing immunoaffinity chromatography of optically active bufuralol and its 1′-oxidized metabolites. Spleen cells from a BALB/c mouse, which had been immunized with the (1R)- or (1S)-1′-oxobufuralol O-carboxymethyl oxime-bovine serum albumin conjugate, were fused with P3/NS1/1-Ag4-1 myeloma cells. After screening by enzyme-linked immunosorbent assay (ELISA) using a β-galactosidase-labeled antigen, one for (1R)-bufuralol and three kinds for (1S)-bufuralol of antibody-secreting hybridoma clones were established. The monoclonal antibodies, Ab-R-12 and Ab-S-1,were determined to have favorable characteristics for preparing the immunosorbent, because each of them was specific for (1R)- or (1S)-bufuralols and its corresponding 1′-oxidized metabolites but showed significantly low cross-reactivities with their antipodes including 1′-oxidized metabolites.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 16 (1), 31-35, 2000
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679232432256
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- NII論文ID
- 10004704582
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3cXos1ektA%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4966599
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可