Detection and Imaging of L-Glutamate Released from Mouse-Brain Slices with an Enzyme-Based Membrane.

DOI PDF Web Site Web Site Web Site 被引用文献6件 参考文献43件
  • HIRANO Ayumi
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • ASAKAWA Minako
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • KIDO Naoko
    Department of Chemistry, College of Humanities and Sciences, Nihon University
  • SUGAWARA Masao
    Department of Chemistry, College of Humanities and Sciences, Nihon University

この論文をさがす

抄録

A new and simple method for detecting and color imaging of L-glutamate released in mouse-brain slices is described based on an enzyme-coupling reaction. The method utilizes L-glutamate oxidase (GluOx) and horseradish peroxidase (HRP) immobilized in a bovine serum albumin (BSA)-glutaraldehyde matrix on a poly-L-lysine-coated glass cover slip. The enzymes are spontaneously and continuously supplied from the membrane into an extracellular solution of the brain slice placed on the membrane after dipping in a redox substrate (DA-64). The Bindschedler’s Green (BG) converted from the DA-64 by the enzyme reaction gives green signals in the brain slice, which are related to the concentration of L-glutamate released in its regions. The first examples of color images for the spatial distribution of L-glutamate in the regions of adult mouse brain slices are demonstrated. The regions where the intense green signals were observed matched the reported distribution of mRNAs encoding the NMDA receptor in the mouse-brain slice.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 16 (1), 25-29, 2000

    社団法人 日本分析化学会

被引用文献 (6)*注記

もっと見る

参考文献 (43)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ