Novel Chromogenic Substrates for the Rate-Assay of N-Acetyl-.BETA.-D-glucosaminidase: Resorufinyl- and Resazurinyl-N-acetyl-.BETA.-D-glucosaminides.

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  • Novel Chromogenic Substrates for the Rate-Assay of N-Acetyl-β-D-glucosaminidase: Resorufinyl- and Resazurinyl-N-acetyl-β-D-glucosaminides

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Two novel Nacetyl-β-D-glucosaminides, resorufinyl-(1) and resazurinyl-Nacetyl-β-D-glucosaminides (2), were synthesized by a coupling reaction of 1-chloro-l-deoxy-2, 3, 4, 6-tetraacetyl-α-D-glucosamine with resorufin derivatives followed by deprotection of the sugar moiety. Compounds 1 and 2 were hydrolyzed by N-acetyl-β-D-glucosaminidase to give products showing high absorbance at a long absorption wavelength (575 and 610nm) under rate-assay conditions of a weakly acidic pH. Though these two compounds are considered to be potential chromogenic substrates, 1 is the more favorable substrate for the rate-assay of N-acetyl-β-D-glucosaminidase.

Journal

  • Analytical Sciences

    Analytical Sciences 8 (2), 161-164, 1992

    The Japan Society for Analytical Chemistry

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