Screening of Endocrine Disrupting Chemicals Using a Surface Plasmon Resonance Sensor
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- ASANO Kazunobu
- New Business Development, Biacore K.K.
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- ONO Atsushi
- New Business Development, Biacore K.K.
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- HASHIMOTO Setsuko
- New Business Development, Biacore K.K.
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- INOUE Tohru
- New Business Development, Biacore K.K.
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- KANNO Jun
- New Business Development, Biacore K.K.
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Because concern over endocrine disrupting reactions caused by chemicals to humans and animals is growing, a rapid and reliable screening assay for endocrine disrupting chemicals is required. We have developed an in vitro screening assay based on a hormone receptor mechanism using a surface plasmon resonance (SPR) sensor. The interaction between an estrogen receptor α(ER) and an estrogen response element (ERE) is monitored in real time, when ER is injected over the SPR sensor chip on which a DNA fragment containing ERE is immobilized. In the presence of a chemical with estrogenic activity, the ER-ERE interaction is enhanced and the kinetic parameters are altered. We have validated the assay in terms of its specificity, dose dependency, optimal reaction conditions and reproducibility. It has been shown that the assay is very reliable as a rapid and quantitative screening method to judge the estrogenic activities of chemicals.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 20 (4), 611-616, 2004
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679232576640
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- NII論文ID
- 10012850555
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXjsVGltLs%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6911950
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- PubMed
- 15116956
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- Web Site
- http://id.ndl.go.jp/bib/6911950
- https://ndlsearch.ndl.go.jp/books/R000000004-I6911950
- https://link.springer.com/content/pdf/10.2116/analsci.20.611.pdf
- https://link.springer.com/article/10.2116/analsci.20.611/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/20/4/20_4_611/_pdf
- https://search.jamas.or.jp/link/ui/2005259935
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
- CiNii Articles
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