Application of Polyaniline/Sol-Gel Derived Tetraethylorthosilicate Films to an Amperometric Lactate Biosensor

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  • CHAUBEY Asha
    Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory
  • PANDE K. K.
    Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory
  • MALHOTRA B. D.
    Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory

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Abstract

The electrochemical entrapment of polyaniline (PANI) onto sol-gel derived tetraethylorthosilicate (TEOS) films deposited onto indium-tin-oxide (ITO) coated glass has been utilized for immobilization of lactate dehydrogenase (LDH). The performance of these sol-gel/PANI/LDH electrodes has been investigated as a function of the lactate concentration, applied potential, pH of the medium and interferents. The amperometric response of the electrodes under optimum conditions exhibited a linear relationship from 1 mM to 4 mM. An attempt has been made to extend the linearity up to 10 mM for lactate by coating an external layer of polyvinyl chloride (PVC) over the sol-gel/PANI/LDH electrodes with a correlation coefficient of 0.89. These sol-gel/PANI/LDH electrodes have a response time of about 60 s, a shelf life of about 8 weeks at 0 - 4°C and have implications in a lactate biosensor.

Journal

  • Analytical Sciences

    Analytical Sciences 19 (11), 1477-1480, 2003

    The Japan Society for Analytical Chemistry

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