Measurement of Transport Activities of Bile Acids in Human Multidrug Resistance-Associated Protein 3 Using Liquid Chromatography-Tandem Mass Spectrometry
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- YAMAGUCHI Kana
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- MURAI Tsuyoshi
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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- YABUUCHI Hikaru
- Office of Collaborative Research and Technology Development, Kobe University
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- KUROSAWA Takao
- Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido
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Description
A method has been developed for the measurement of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 3-expressing Sf9 cells for 1β-hydroxy-, 6α-hydroxy- and unsaturated bile acids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10 to 2000 pmol/mL, and the detection limit was less than 2 pmol/mL for all bile acids using selected reaction monitoring analysis. The method was applied to measurements of adenosine triphosphate-dependent transport activities of the membrane vesicles for the above-mentioned hydroxylated and unsaturated bile acids. The present study demonstrated that the human multidrug resistance-associated protein 3 vesicles accepted 1β-, 6α-hydroxylated and unsaturated bile acids along with common bile acids, such as glycocholic acid and taurolithocholic acid 3-sulfate. The developed method is useful for measurements of bile acid transport activities.
Journal
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- Analytical Sciences
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Analytical Sciences 26 (3), 317-323, 2010
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679232784384
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- NII Article ID
- 10026782326
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10585586
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed