Electrochemical Gene Sensor Arrays Prepared Using Non-contact Nanoliter Array Spotting of Gene Probes

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  • AOKI Hiroshi
    National Institute of Advanced Industrial Science and Technology (AIST)
  • KITAJIMA Akiko
    National Institute of Advanced Industrial Science and Technology (AIST)
  • TAO Hiroaki
    National Institute of Advanced Industrial Science and Technology (AIST)

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Abstract

A capillary-based pitch-variable array spotter, designed to dispense solutions in nanoliter volumes with high precision and density, was used to immobilize gene probes on a microelectrode array chip. Small volumes of the probe solutions were dispensed onto the microelectrodes, without any physical contact between the capillary ends and the electrode surfaces, to fabricate an electrochemical gene sensor array chip. The fabricated gene sensor array chip showed sequence-selective responses, expressed on a pseudocolor scale, to a target DNA sample. It was demonstrated that this dispensing technique provides integrated sensor array chips by dispensing small volumes of solutions of synthesized functional gene probes onto microelectrode array chips, a process not possible with conventional dispensing techniques.

Journal

  • Analytical Sciences

    Analytical Sciences 26 (3), 367-370, 2010

    The Japan Society for Analytical Chemistry

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