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Electrochemical Gene Sensor Arrays Prepared Using Non-contact Nanoliter Array Spotting of Gene Probes
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- AOKI Hiroshi
- National Institute of Advanced Industrial Science and Technology (AIST)
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- KITAJIMA Akiko
- National Institute of Advanced Industrial Science and Technology (AIST)
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- TAO Hiroaki
- National Institute of Advanced Industrial Science and Technology (AIST)
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Description
A capillary-based pitch-variable array spotter, designed to dispense solutions in nanoliter volumes with high precision and density, was used to immobilize gene probes on a microelectrode array chip. Small volumes of the probe solutions were dispensed onto the microelectrodes, without any physical contact between the capillary ends and the electrode surfaces, to fabricate an electrochemical gene sensor array chip. The fabricated gene sensor array chip showed sequence-selective responses, expressed on a pseudocolor scale, to a target DNA sample. It was demonstrated that this dispensing technique provides integrated sensor array chips by dispensing small volumes of solutions of synthesized functional gene probes onto microelectrode array chips, a process not possible with conventional dispensing techniques.
Journal
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- Analytical Sciences
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Analytical Sciences 26 (3), 367-370, 2010
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679232813056
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- NII Article ID
- 130004441959
- 10026782541
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- NII Book ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 10585660
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- PubMed
- 20215688
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed