Ratiometric Fluorescence Detection of Pyrimidine/Purine Transversion by Using a 2-Amino-1,8-naphthyridine Derivative
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- SATAKE Hiroyuki
- Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
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- NISHIZAWA Seiichi
- Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
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- TERAMAE Norio
- Department of Chemistry, Graduate School of Science, Tohoku University, and CREST, Japan Science and Technology Agency (JST)
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抄録
A new class of abasic site-binding fluorescence ligands, Naph-NBD in which 7-nitrobenzo-2-oxa-1,3-diazole (NBD) is connected to 2-amino-7-methyl-1,8-naphthyridine (Naph) by a propylene linker, is presented for the ratiometric assay for SNPs typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), Naph-NBD is found to selectively recognize pyrimidine bases over purine bases opposite the abasic site in DNA duplexes (K11/M-1: T, 8.1 × 106; C, 2.5 × 106; G, 0.33 × 106; A, 0.27 × 106). The binding of Naph-NBD is accompanied by significant quenching of the fluorescence from the naphthyridine moiety (λmax, 409 nm), while the emission from the NBD (λmax, 544 nm) is relatively unaffected. Such a fluorescence response of Naph-NBD allows the emission ratio detection of pyrimidine/purine transversion.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 22 (2), 195-197, 2006
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233051008
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- NII論文ID
- 130004441234
- 10017246151
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD28XitFeru7k%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 7806554
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- PubMed
- 16512406
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可