Microcantilevers Modified by Horseradish Peroxidase Intercalated Nano-Assembly for Hydrogen Peroxide Detection

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  • YAN Xiaodong
    Institute for Micromanufacturing, Louisiana Tech University
  • SHI Xiaolei
    Institute for Micromanufacturing, Louisiana Tech University
  • HILL Kalisha
    Institute for Micromanufacturing, Louisiana Tech University
  • JI Hai-Feng
    Institute for Micromanufacturing, Louisiana Tech University

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抄録

Detection of hydrogen peroxide is of industry interest and of biological importance. Here we report a new approach to hydrogen peroxide measurement using multilayer modified microcantilevers. Through a layer-by-layer nanoassembly technique, horseradish peroxidase was intercalated into a nanoscale multilayer assembly on one surface of microcantilevers. These enzyme-functionalized microcantilevers deflected in response to hydrogen peroxide concentrations in the nanomolar level. The magnitudes of bending were proportional to the concentrations of hydrogen peroxide. Furthermore, our study also showed that microcantilever technique may be used as a novel, more sensitive tool for the study of the conformational or structural changes of enzymes or proteins on surfaces.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 22 (2), 205-208, 2006

    社団法人 日本分析化学会

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