Microcantilevers Modified by Horseradish Peroxidase Intercalated Nano-Assembly for Hydrogen Peroxide Detection

DOI PDF Web Site Web Site Web Site 62 References
  • YAN Xiaodong
    Institute for Micromanufacturing, Louisiana Tech University
  • SHI Xiaolei
    Institute for Micromanufacturing, Louisiana Tech University
  • HILL Kalisha
    Institute for Micromanufacturing, Louisiana Tech University
  • JI Hai-Feng
    Institute for Micromanufacturing, Louisiana Tech University

Search this article

Abstract

Detection of hydrogen peroxide is of industry interest and of biological importance. Here we report a new approach to hydrogen peroxide measurement using multilayer modified microcantilevers. Through a layer-by-layer nanoassembly technique, horseradish peroxidase was intercalated into a nanoscale multilayer assembly on one surface of microcantilevers. These enzyme-functionalized microcantilevers deflected in response to hydrogen peroxide concentrations in the nanomolar level. The magnitudes of bending were proportional to the concentrations of hydrogen peroxide. Furthermore, our study also showed that microcantilever technique may be used as a novel, more sensitive tool for the study of the conformational or structural changes of enzymes or proteins on surfaces.

Journal

  • Analytical Sciences

    Analytical Sciences 22 (2), 205-208, 2006

    The Japan Society for Analytical Chemistry

References(62)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top