Photochemical fishing approaches for identifying target proteins and elucidating the structure of a ligand-binding region using carbine-generating photoreactive probes
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- SADAKANE Yutaka
- Faculty of Pharmaceutical Sciences, Kyushu University of Health and Welfare
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- HATANAKA Yasumaru
- Faculty of Pharmaceutical Sciences, University of Toyama
書誌事項
- タイトル別名
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- Photochemical Fishing Approaches for Identifying Target Proteins and Elucidating the Structure of a Ligand-binding Region Using Carbene-generating Photoreactive Probes
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説明
Photoaffinity labeling enables the direct probing of a target protein through a covalent bond between a ligand and its binding protein, and even a complex formed by weak interactions can be isolated by the method. The photochemical fishing approach accelerates the throughput, isolating crosslinked complexes and analyzing the structure of the ligand binding site within the protein. We used carbene-generating phenyldiazirine for this approach because practical examinations had shown that the phenyldiazirine functioned as the powerful barb on the hook. Improving the synthetic pathways of the photoprobes and using chemoselective-integrated photoreactive units makes possible the easy and rapid preparation of carbene-generating photoreactive probes including the derivatives in peptides, proteins, DNAs, and carbohydrates. This review also shows several recent impacts of photoaffinity labeling, including the in vivo preparation of photoreactive proteins in living cells.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 22 (2), 209-218, 2006
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233055232
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- NII論文ID
- 130004441238
- 10017246234
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD28XitFeqsr8%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 7806596
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可