Nitric Oxide Biosensors Based on Hb/Phosphatidylcholine Films.

FAN Chunhai, PANG Jiantao, SHEN Pingping, LI Genxi, ZHU Dexu

doi:10.2116/analsci.18.129

Nitric Oxide Biosensors Based on Hb/Phosphatidylcholine Films.

DOI PDF Web Site Web Site Web Site PubMed ほか1件をすべて表示一部だけ表示被引用文献2件参考文献39件

FAN Chunhai

Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University
PANG Jiantao

Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University
SHEN Pingping

Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University
LI Genxi

Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University
ZHU Dexu

Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University

この論文をさがす

抄録

Hemoglobin (Hb) was entrapped in a phosphatidylcholine (PC) film and immobilized at a pyrolytic graphite (PG) electrode surface. Its electron-transfer reactivity and enzyme activity were characterized by employing electrochemical methods. It was observed that Hb exhibited direct electrochemistry as well as enzyme-like activity towards the electro-catalytic reduction of NO in PC film. An unmediated, reagentless nitrogen oxide (NO) biosensor was accordingly prepared. Experimental results revealed that the peak current related to NO was linearly proportional to its concentration in the range of 1.0 × 10^-7 - 3.0 × 10^-4 mol/L. The detection limit was estimated to be 1.0 × 10^-7 mol/L. Considering its good stability, nice selectivity and easy construction, this biosensor shows great promise for the rapid determination of traces of NO.