A New Spectrofluorometric Method for the Determination of Ascorbic Acid Based on Its Activating Effect on a Hemoglobin-Catalyzed Reaction.

DOI PDF Web Site Web Site Web Site View 1 Remaining Hide 22 References

Bibliographic Information

Other Title
  • New Spectrofluorometric Method for the Determination of Ascorbic Acid Based on Its Activating Effect on a Hemoglobin Catalyzed Reaction

Search this article

Description

A spectrofluorometric method for the determination of ascorbic acid (AA) based on its activation on the hemoglobin-catalyzed reaction was proposed. The fluorescence intensity of the product was measured under the optimal experimental conditions, i.e. 4.0 × 10-6 M H2O2, 6.0 × 10-5 M p-cresol, 1.2 M NH3-NH4Cl (pH 10.4) and 2.0 × 10-7 M hemoglobin. The order of additions of the reagents was also studied. The activation of AA was found to be associated with a high ammonia concentration. The linear range of the method was 9.0 × 10-10 - 3.6 × 10-8 M of AA. The detection limit was calculated to be 3.0 × 10-10 M. The relative standard deviation of this method is 1.6% at 7.0 × 10-9 M for 11 determinations.

Journal

  • Analytical Sciences

    Analytical Sciences 18 (9), 977-980, 2002

    The Japan Society for Analytical Chemistry

References(22)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top