A Peptide Microarray for the Detection of Protein Kinase Activity in Cell Lysate

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  • SHIGAKI Syuhei
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University
  • YAMAJI Takayuki
    Graduate School of Systems Life Sciences, Kyushu University
  • HAN Xiaoming
    Graduate School of Systems Life Sciences, Kyushu University
  • YAMANOUCHI Go
    Graduate School of Systems Life Sciences, Kyushu University
  • SONODA Tatsuhiko
    Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology
  • OKITSU Osamu
    Applied Genomics, Molecular Medicine Research Laboratories, Astellas Pharmaceuticals Inc.
  • MORI Takeshi
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
  • NIIDOME Takuro
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University
  • KATAYAMA Yoshiki
    Department of Applied Chemistry, Faculty of Engineering, Kyushu University Center for Future Chemistry, Kyushu University

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  • Peptide Microarray for the Detection of Protein Kinase Activity in Cell Lysate

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Description

DNA microarray enables the analysis of DNA or mRNA expression levels, but it has not been possible to completely understand life using obtained information. Consequently, protein or peptide arrays have attracted much interest. Since the development of a practical protein microarray is still far away in light of handling difficulties, the peptide microarray is a promising tool for analyzing protein functions. We have developed a peptide microarray to detect protein kinase activity in cell lysate. All substrate peptides for kinases were immobilized chemoselectively on amino-coated glass slides. After phosphorylation of the immobilized peptides, phosphorylation was detected by fluorescence imaging. We detected the protein kinase activities, including that in cell lysate, in response to drug stimulation. Therefore, this peptide microarray would be useful for a high-throughput kinase assay of intracellular signals and would be applicable to drug screening.

Journal

  • Analytical Sciences

    Analytical Sciences 23 (3), 271-275, 2007

    The Japan Society for Analytical Chemistry

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