Measurement of Mouse Urinary Type 4 Collagen Using Time-Resolved Fluoroimmunoassay

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  • KOBAYASHI Michimasa
    Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine
  • KIMURA Hiroko
    Department of Forensic Medicine, Juntendo University School of Medicine
  • LIAO Jie
    Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine
  • ABE Masaaki
    Department of Pathology, Juntendo University School of Medicine
  • HIROSE Sachiko
    Department of Pathology, Juntendo University School of Medicine
  • TOMINO Yasuhiko
    Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine

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タイトル別名
  • Measurement of Mouse Urinary Type IV Collagen Using Time-Resolved Fluoroimmunoassay.

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抄録

Urinary level of type IV collagen is an important indicator for early renal dysfunction, but there has been no practical system to measure mouse type IV collagen adaptable to extremely small amounts of urine samples. We developed a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) to measure mouse urinary type IV collagen. Based on the structural features of type IV collagen molecule, dithiothreitol (DTT) was used for pretreatment of the samples. This assay permits measurement of 100 pg/ml type IV collagen in 5 μl urine samples. Urinary levels of type IV collagen derived from 12 samples of two different mouse strains (KK/Ta and BALB/c) were measured using this assay. The results demonstrated very clearly the difference in values of urinary type IV collagen between diabetic mice and non-diabetic mice. Compared with the conventional enzyme-linked immunosorbent assay (ELISA), this method requires far smaller volumes of samples, and is best suited to mouse models in future experiments.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 19 (2), 205-210, 2003

    社団法人 日本分析化学会

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