Rapid Determination of Chloramphenicol Residues in Aquaculture Tissues by Immunochromatographic Assay

LI Ke, LIU Liqiang, XU ChuanLai, CHU XiaoGang

doi:10.2116/analsci.23.1281

Rapid Determination of Chloramphenicol Residues in Aquaculture Tissues by Immunochromatographic Assay

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LI Ke

WuXi KangBaiEr Biotechnology Corporation
LIU Liqiang

School of Food Science and Technology, Southern Yangtze University
XU ChuanLai

School of Food Science and Technology, Southern Yangtze University
CHU XiaoGang

Chinese Academy of Inspection and Quarantine

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抄録

An immunochromatographic assay was developed to detect chloramphenicol (CAP) residues in aquaculture tissues. The limit of detection (LOD) was 10 ng g^-1 for detecting CAP spiked in the aquaculture tissues. The results were confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS) and indicated that there was a good agreement between the two methods. The linear regression equation was y = 1.19x + 0.539 with R² = 0.978. The assay time for test was less than 5 min and the method is suitable for rapid testing on-site.