Separation of 2-Aminobenzoic Acid-Derivatized Glycosaminoglycans and Asparagine-linked Glycans by Capillary Electrophoresis

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  • SATO Kae
    Department of Applied Biological Chemistry, University of Tokyo
  • SATO Kiichi
    Department of Applied Biological Chemistry, University of Tokyo
  • OKUBO Akira
    Department of Applied Biological Chemistry, University of Tokyo
  • YAMAZAKI Sunao
    Department of Applied Biological Chemistry, University of Tokyo

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Abstract

A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan Δ-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. This analytical method was applied to the identification of glycosaminoglycan in combination with enzymatic digestion. The separation of N-glycans or glucose-oligomers was performed with a phosphate buffer containing polyethylene glycol or borate as an electrolyte solution. This method is expected to be useful in the determination of oligosaccharide structures in a glycoprotein.

Journal

  • Analytical Sciences

    Analytical Sciences 21 (1), 21-24, 2005

    The Japan Society for Analytical Chemistry

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