Adsorption Behavior of Cytochrome c, Myoglobin and Hemoglobin in a Quartz Surface Probed Using Slab Optical Waveguide (SOWG) Spectroscopy.
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- SANTOS Jose H.
- Nanoarchitectonics Research Center, AIST
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- MATSUDA Naoki
- Nanoarchitectonics Research Center, AIST
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- QI Zhi-mei
- Nanoarchitectonics Research Center, AIST
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- YOSHIDA Takamitsu
- Nanoarchitectonics Research Center, AIST Institute of Materials Science, Tsukuba University
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- TAKATSU Akiko
- National Metrology Institute of Japan
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- KATO Kenji
- National Metrology Institute of Japan
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Abstract
Slab optical waveguide (SOWG) spectroscopy was used to observe the adsorption behavior of three important heme proteins, namely cytochrome c, myoglobin and hemoglobin, in a quartz surface. Using prism-coupled polychromatic visible light propagated into a quartz waveguide by internal total reflection, the real-time monitoring of evanescent wave absorption revealed a strong dependence of the protein-surface interaction on the protein concentration, the solution pH and the ionic strength. For the three proteins studied, the absorbance-bulk concentration ratio was higher at low bulk concentrations, and decreased at higher concentrations. For cytochrome c and myoglobin, the absorbance approached a limiting value, but buffered hemoglobin surprisingly did not show any indication of forming a signal plateau. Moreover, the slow introduction of protein into the solution lessened the total adsorbed amount per unit area. These observations suggested a possible conformational transition of the protein molecules at the quartz surface after adsorption. For a bulkier protein, hemoglobin, adsorption onto the quartz surface was enhanced in the presence of a phosphate buffer, while the opposite effect was observed for the smaller cytochrome c and myoglobin molecules. The results of pH studies concurred with the electrostatic interactions predicted from the isoelectric data of proteins and the quartz surface.
Journal
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- Analytical Sciences
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Analytical Sciences 19 (2), 199-204, 2003
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679233348096
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- NII Article ID
- 130004440697
- 50000738982
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3sXhtlOgtrg%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6442807
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- PubMed
- 12608745
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- Web Site
- http://id.ndl.go.jp/bib/6442807
- https://ndlsearch.ndl.go.jp/books/R000000004-I6442807
- https://link.springer.com/content/pdf/10.2116/analsci.19.199.pdf
- https://link.springer.com/article/10.2116/analsci.19.199/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/19/2/19_2_199/_pdf
- https://search.jamas.or.jp/link/ui/2003221288
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed