Comparison of Enzymatic Recycling Electrodes for Measuring Aminophenol: Development of a Highly Sensitive Natriuretic Peptide Assay System

MIE Yasuhiro, KOWATA Keiko, HIRANO Yu, NIWA Osamu, MIZUTANI Fumio

doi:10.2116/analsci.24.577

Comparison of Enzymatic Recycling Electrodes for Measuring Aminophenol: Development of a Highly Sensitive Natriuretic Peptide Assay System

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MIE Yasuhiro

Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
KOWATA Keiko

Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
HIRANO Yu

Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)
NIWA Osamu

Tsukuba Center, National Institute of Advanced Industrial Science and Technology (AIST)
MIZUTANI Fumio

Hokkaido Center, National Institute of Advanced Industrial Science and Technology (AIST)

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抄録

Several redox enzymes were examined for enzymatic/electrochemical-recycling systems in order to measure p-aminophenol (PAP) with high sensitivity. Glucose oxidase (GOD) and diaphorase (DI) worked well as catalysts for recycling electrode systems: these enzymes effectively reduced p-iminoquinone (PIQ), the electrochemically-oxidized form of PAP, and caused an enhancement in the electrochemical signals (anodic currents in the voltammogram and amperogram) by ∼ 100 fold. The lower detection limits for PAP were estimated to be 50 nM with the GOD system and 2 nM with the DI system. We combined the enzymatic-recycling electrode using DI with an enzyme immunoassay system to measure atrial natriuretic peptide (ANP), an important marker peptide hormone involved in heart diseases. ANPs from serum samples at ppt-levels were determined appropriately using the present assay system.