Analysis of calmodulin fragments and preparation of the peptides containing calcium binding domains by high performance liquid chromatography.

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  • KAWASAKI Hiroshi
    Present address: The Tokyo Metropolitan Institute of Medical Science
  • KUROSU Yasuyuki
    Present address: Japan Spectroscopic Co., Ltd.
  • KASAI Hisataka
    Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
  • OKUYAMA Tsuneo
    Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
  • ISOBE Toshiaki
    Department of Chemistry, Faculty of Science, Tokyo Metropolitan University

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Description

The degradation process of calmodulin with trypsin in the presence or absence of calcium ions was reinvestigated by the analysis on HPLC. All tryptic peptides and intermediate peptides were mapped on this system. On the basis of these results, the strategy of the preparation of various calmodulin fragments was devised. Twelve peptides which contain calcium binding sites were obtained.

Journal

  • Analytical Sciences

    Analytical Sciences 2 (3), 287-291, 1986

    The Japan Society for Analytical Chemistry

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