Enzyme-Release Capillary as a Facile Enzymatic Biosensing Part for a Capillary-Assembled Microchip

HENARES Terence G., MAEKAWA Eiji, OKUBO Fumito, MIZUTANI Fumio, YAO Toshio, SEKIZAWA Ryuichi, HISAMOTO Hideaki

doi:10.2116/analsci.25.1025

Enzyme-Release Capillary as a Facile Enzymatic Biosensing Part for a Capillary-Assembled Microchip

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HENARES Terence G.

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
MAEKAWA Eiji

Graduate School of Material Science, University of Hyogo
OKUBO Fumito

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
MIZUTANI Fumio

Graduate School of Material Science, University of Hyogo
YAO Toshio

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University
SEKIZAWA Ryuichi

Metaboscreen Co. Ltd.
HISAMOTO Hideaki

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University

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抄録

A simple capillary enzymatic biosensor was developed. This was prepared by simply coating a dissolvable membrane containing enzyme/s on the inner wall of a square glass capillary. An easy measurement was carried out by capillary force sample introduction with concurrent enzyme release and a reaction with a certain substrate. Enzyme-release capillary (ERC) biosensors showed long-term storage stability of at least two weeks for a β-galactoside derivative and glucose. Moreover, this could be integrated on a capillary-assembled microchip (CAs-CHIP) to broaden its multiple analyte sensing potential for clinical diagnostic applications.