Investigation of the Chemico-physical Characteristics of the Active Components in the Chinese Herb Gastrodia elata Bl. by Capillary Zone Electrophoresis.

WANG Dexian, YANG Gengliang, LI Baohui, LI Zhiwei, CHEN Yi

doi:10.2116/analsci.18.409

Investigation of the Chemico-physical Characteristics of the Active Components in the Chinese Herb Gastrodia elata Bl. by Capillary Zone Electrophoresis.

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WANG Dexian

Department of Chemistry, Hebei University Hebei Medical College for Continue Education
YANG Gengliang

Department of Chemistry, Hebei University Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science
LI Baohui

Department of Chemistry, Hebei University
LI Zhiwei

Department of Chemistry, Hebei University
CHEN Yi

Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science

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抄録

A CE method for investigating the chemico-physical characteristics of the active components of low molecular weight in Gastrodia elata Bl. is described. First, the dissociation constants of five active components were determined based on the relation between the effective mobility of the solutes and the buffer pH value. Second, an equation that describes the relation of the migration time and the molecular weight was developed and used to predict the migration order and to calculate the electroosmotic velocity. The results predicted by theory agreed well with that from experiments.