A Simple HPLC-Fluorescence Detection of Nitric Oxide in Cultivated Plant Cells by in situ Derivatization with 2,3-Diaminonaphthalene.
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- WADA Mitsuhiro
- School of Pharmaceutical Sciences, Nagasaki University
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- MORINAKA Chieko
- School of Pharmaceutical Sciences, Nagasaki University
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- IKENAGA Toshihiko
- Faculty of Environmental Studies, Nagasaki University
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- KURODA Naotaka
- School of Pharmaceutical Sciences, Nagasaki University
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- NAKASHIMA Kenichiro
- Graduate School of Pharmaceutical Sciences, Nagasaki University
書誌事項
- タイトル別名
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- Simple HPLC Fluorescence Detection of Nitric Oxide in Cultivated Plant Cells by in situ Derivatization with 2 3 Diaminonaphthalene
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An HPLC method with fluorescence detection for the determination of nitric oxide (NO) in cultivated plant cells (Agave pacifica, Agavaceae) was developed. NO was derivatized in situ with 2,3-diaminonaphthalene (DAN) as a labeling reagent and converted to 1(H)-naphthotriazole. The maximum peak height of the derivative was observed by incubation for 3 h at 25°C with 0.2 mM DAN. Excess reagent in cells was removed by washing 3 times with 5 ml of water. The calibration curve for authentic standard of DAN-NO spiked to cultivated plant cells showed a good linearity (r = 0.995) in the range of 5.0 to 50 pmol/g cell. The detection limit at a signal-to-noise ratio of 3 was 3.4 pmol/g cells. The proposed method was successfully applied to the monitoring of NO concentration with cell growth. The effect of thermal treatment on the concentration of NO in plant cells was also examined. The concentration of NO in cells treated at 5°C for 1 h was significantly higher than that treated at 25°C and 35°C for 1 h (n = 3, p < 0.05).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 18 (6), 631-634, 2002
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233565184
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- NII論文ID
- 130004440621
- 10011312429
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38Xks12htbY%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 6185084
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- PubMed
- 12083545
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可