Elimination of Ingredients Effect to Improve the Detection of Anti HIV-1 p24 Antibody in Human Serum Using SPR Apparatus.
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- HIFUMI Emi
- Hiroshima Prefectural University, School of Biosciences
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- KUBOTA Naruhiko
- Hiroshima Prefectural University, School of Biosciences
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- NIIMI Yoshiyuki
- Hiroshima Prefectural University, School of Biosciences
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- SHIMIZU Kosuke
- Hiroshima Prefectural University, School of Biosciences
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- EGASHIRA Naoyoshi
- Hiroshima Prefectural University, School of Biosciences
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Abstract
Highly sensitive detection of proteins in serum becomes difficult in some cases during surface plasmon resonance (SPR) measurements, because some ingredients in the serum hugely enhance non-specific reactions on the sensing chip of SPR. It is well recognized that the antibody against core protein p24 of HIV in serum is one of the most important proteins in the accurate diagnosis of infection with HIV. In this study, we could attain the accurate detection of anti p24 antibody in human serum by eliminating the serious effects of the ingredients in serum on the measurement of SPR by employing these procedures: 1) blocking the gold surface of the sensing chip with human serum and 2) heating the serum sample at 56°C for 30 min. Without these treatments, the signal of SPR was considerably suppressed on the measurement of the anti p24 antibody which contained human serum, making the accurate detection difficult. However, with introducing the above two treatments, the sensing of anti p24 antibody in human serum was improved, while a small non-specific reaction was still observed. By removing the non-specific reaction caused by the ingredients in the serum, we could accurately measure the antibody for p24 in human serum sample over the range from 1 to 20 μg/ml.
Journal
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- Analytical Sciences
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Analytical Sciences 18 (8), 863-867, 2002
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390282679233581824
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- NII Article ID
- 10011311336
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD38Xmtl2jsbw%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6261505
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- PubMed
- 12200830
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- Web Site
- http://id.ndl.go.jp/bib/6261505
- https://ndlsearch.ndl.go.jp/books/R000000004-I6261505
- https://link.springer.com/content/pdf/10.2116/analsci.18.863.pdf
- https://link.springer.com/article/10.2116/analsci.18.863/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/18/8/18_8_863/_pdf
- https://search.jamas.or.jp/link/ui/2003033520
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed