A Sensitive Chemiluminescence Method for the Determination of H<sub>2</sub>O<sub>2</sub> in Exhaled Breath Condensate

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  • HU Yufei
    Department of Chemistry, Institute of Analytical Science, Southwest University
  • ZHANG Zhujun
    Department of Chemistry, Institute of Analytical Science, Southwest University
  • YANG Chunyan
    Department of Chemistry, Institute of Analytical Science, Southwest University

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  • A sensitive chemiluminescence method for the determination of H2O2 in exhaled breath condensate

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Abstract

In this paper, a novel flow injection chemiluminescence (FI-CL) method is proposed for the determination of picomolar L-1 levels of hydrogen peroxide (H2O2) in exhaled breath condensate (EBC). This method is based on the oxidation of a low concentration of luminol (10-7 M) by H2O2 at a low concentration level (< 10-8 M) in an alkaline medium catalyzed by a complex, K5[Cu(HIO6)2] (DPC), which is not interfered by other metal ions or horseradish peroxidase (HRP). Under the optimum conditions, H2O2 was determined over the range of 1.0 × 10-10 to 1.0 × 10-8 mol L-1 with a detection limit of (3σ) of 4.1 × 10-11 mol L-1. The relative standard deviation (RSD) was 3.2% for 5 nmol L-1 H2O2 (n = 7). The proposed method offers the advantages of ultra-sensitivity, selectivity, simplicity and rapidity for H2O2 determination. It was successfully applied to directly determine trace amounts of H2O2 (nmol L-1) in human's EBC of both rheum and healthy volunteers. A statistically significant difference was found between patients with rheum (n = 11) and control subjects without rheum (n = 11).

Journal

  • Analytical Sciences

    Analytical Sciences 24 (2), 201-205, 2008

    The Japan Society for Analytical Chemistry

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