Nuclease Tolerant FRET Probe Based on DNA-Quantum Dot Conjugation

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  • ONOSHIMA Daisuke
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
  • KAJI Noritada
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
  • TOKESHI Manabu
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
  • BABA Yoshinobu
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University Plasma Nanotechnology Research Center, Nagoya University Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST) Institute for Molecular Science, National Institutes of Natural Sciences

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Abstract

We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).

Journal

  • Analytical Sciences

    Analytical Sciences 24 (2), 181-183, 2008

    The Japan Society for Analytical Chemistry

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