Determination of Urea in Serum Based on the Combination of an Enzymatic Reaction with Immobilized Urease and Ion Chromatographic Analysis
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- MIYAUCHI Toshiyuki
- Department of Applied Chemistry, College of Engineering, Chubu University
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- MIYACHI Yasutaka
- Department of Applied Chemistry, College of Engineering, Chubu University
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- TAKAHASHI Makoto
- Department of Applied Chemistry, College of Engineering, Chubu University
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- ISHIKAWA Norihisa
- Department of Applied Chemistry, College of Engineering, Chubu University
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- MORI Hidehiko
- Department of Applied Chemistry, College of Engineering, Chubu University
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A quantitative method for the determination of urea in serum was studied. An ion chromatograph (IC) with a conductivity detector was used in this method, where the chromatograph was modified by placing an immobilized urease column between the injection loop and a guard column of the cation analysis column. Immobilized urease was prepared by the adsorption of urease on cedar sawdust with triethylenetetramine. The adsorption capacity of urease was 190 mg g−1, and its activity was 3500 U g−1. The conversion efficiency of urea to ammonium ion was 100%, and the half life of immobilized urease was 60 days. It was possible to use the immobilized urease in a pH range of 3.0 to 9.0, and at temperatures up to 60°C. The determination of urea was attempted by IC attaching an immobilized urease column. The limit of detection of urea was 0.2 mg L−1, and the calibration curves of urea were very linear over 0.8 − 25 mg L−1. The urea concentration in the human serum could be determined with a standard deviation of 0.06 − 0.13 within 5 min after injecting the serum sample.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 26 (8), 847-851, 2010
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679233883904
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- NII論文ID
- 10026784706
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC3cjkt1aitw%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 10779895
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- PubMed
- 20702937
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可