In situ Monitoring of Lysozyme Adsorption onto Bare and Cysteine- or 1-Octadecanethiol-Modified Au Electrodes Using an Electrochemical Quartz-Crystal Impedance System.
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- ZHANG You-yu
- Chemical Research Institute, Hunan Normal University
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- XIE Qing-ji
- Chemical Research Institute, Hunan Normal University
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- ZHOU An-hong
- Chemical Research Institute, Hunan Normal University
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- YAO Shou-zhuo
- Chemical Research Institute, Hunan Normal University
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Abstract
The adsorption of lysozyme from phosphate-buffered saline (pH 7.4) onto bare and cysteine- or 1-octadecanethiol-modified Au electrodes was investigated in situ using an electrochemical quartz-crystal impedance system (EQCIS). The equivalent circuit parameters and resonant frequencies of the piezoelectric quartz-crystal (PQC) resonance, the value of half peak width of the electroacoustic conductance spectrum (DfG1/2) as well as the electrical double-layer capacitance were obtained and analyzed. The adsorption kinetics was analyzed with a scheme of two consecutive reactions occurring at the interface. The amount of adsorbed lysozyme on a hydrophobic surface was greater than that on a hydrophilic one, and a more negative potential brought a larger amount of adsorbed lysozyme. The standard heterogeneous rate constants of ferricyanide/ferrocyanide before and after the lysozyme adsorption were evaluated.
Journal
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- Analytical Sciences
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Analytical Sciences 16 (8), 799-805, 2000
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679233910272
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- NII Article ID
- 10005306949
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3cXmtFygu7o%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 5501441
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed