Determination of Superoxide Scavenging Activity of a Sample Containing Xanthine Oxidase Inhibitor by ESR Spin Trapping.

DOI PDF Web Site Web Site Web Site 32 References
  • KOIDE Tomoko
    Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry
  • NODA Hiroyuki
    Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry
  • LIU Wenwei
    Graduate School of Science and Engineering, Yamagata University
  • OGATA Tateaki
    Graduate School of Science and Engineering, Yamagata University
  • KAMADA Hitoshi
    Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry

Search this article

Abstract

The objective of this research was to determine the true superoxide (O2-) scavenging activity of antioxidants in a sample containing xanthine oxidase (XOD) inhibitor. A previous method involving electron spin resonance (ESR) spin-trapping has generally used 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) as a spin trap and the O2- generation system of hypoxanthine (HPX)-xanthine oxidase (XOD). However, this method has a problem in that the ESR signal intensity of the DMPO spin adduct of O2- (DMPO-OOH) is decreased not only by the reaction of a sample with O2-, but also by the inhibition of XOD. Therefore, the ESR spin-trapping method combined with the SOD addition method was developed as a new experimental technique in this study. The results indicate that the O2- scavenging activity of a sample containing allopurinol as an XOD inhibitor could be determined by using this method.

Journal

  • Analytical Sciences

    Analytical Sciences 16 (10), 1029-1032, 2000

    The Japan Society for Analytical Chemistry

References(32)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top