Evaluation and Application of Liquid Chromatographic Columns Coated with "Intelligent" Ligands(3)Immobilized Enzyme Phospholipid Column

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 被引用文献2件 参考文献26件

書誌事項

タイトル別名
  • Evaluation and Application of Liquid Chromatographic Columns Coated with "Intelligent" Ligands. (III). Immobilized Enzyme Phospholipid Column.

この論文をさがす

説明

Immobilized enzyme columns have been developed for use as high-performance liquid chromatographic enzyme reactors. Enzyme reactors were prepared by immobilizing trypsin or cytochrome-c on phospholipid columns. Dynamic coating was employed to prepare the reactors by recycling a buffer solution containing trypsin or cytochrome-c through a phospholipid-coated column, on which the enzymes were immobilized by hydrophobic binding. The immobilized trypsin column displayed hydrolytic activity which catalyzed the hydrolysis of L-amino acid esters to amino acid. The immobilized cytochrome-c column exhibited oxidation activity which catalyzed N-demethylation of N,N-dimethylaniline, codeine, and dihydrocodeine in the presence of hydrogen peroxide as an oxygenating agent. The enzyme reaction proceeded rapidly in the column; both product and substrate could be separated and detected simultaneously. The immobilized enzyme columns could be readily regenerated using the original phospholipid column by repeating the dynamic coating. These immobilized enzyme columns could be utilized as enzyme reactors in the high-performance liquid chromatographic mode. Complete hydrolysis of amino acid ester was observed with the trypsin column. Demethylation of codeine and of dihydrocodeine were observed with the cytchrome-c column.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 17 (9), 1085-1089, 2001

    社団法人 日本分析化学会

被引用文献 (2)*注記

もっと見る

参考文献 (26)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ