Investigation of C-3 Epimerization Mechanism of 24, 25-Dihydroxyvitamin D3 in Rat Using Liquid Chromatography/Mass Spectrometry.
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- HIGASHI Tatsuya
- Faculty of Pharmaceutical Sciences, Kanazawa University
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- OGASAWARA Aki
- Faculty of Pharmaceutical Sciences, Kanazawa University
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- SHIMADA Kazutake
- Faculty of Pharmaceutical Sciences, Kanazawa University
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Studies on C-3 epimerization of 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] were performed. 3-Epi-24,25(OH)2D3 was found to circulate in the blood of rats administered 24,25(OH)2D3, which was confirmed in comparison with a standard sample based on its chromatographic behavior during inclusion high-performance liquid chromatography using γ-cyclodextrinas a mobile phase additive and data obtained from liquid chromatography (LC)/mass spectrometry (MS) and gas chromatography/MS. The C-3 epimerization mechanism was investigated in in vitro experiments using [3α-2H]-24,25(OH)2D3. The disappearance of deuterium was monitored by LC/MS. As a result, it was clarified that the 3-epimer was formed via the 3-oxo-form as an intermediate. This epimerization was also found to be controlled by the enzyme existing in the liver cytosol fraction and to require NAD and NADPH as coenzymes.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 16 (5), 477-482, 2000
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679234113152
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- NII論文ID
- 130004440281
- 120003406267
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3cXjslCns7k%3D
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- ISSN
- 13482246
- 09106340
- http://id.crossref.org/issn/09106340
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- NDL書誌ID
- 5383479
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- Web Site
- http://hdl.handle.net/2297/29326
- http://id.ndl.go.jp/bib/5383479
- https://ndlsearch.ndl.go.jp/books/R000000004-I5383479
- https://link.springer.com/content/pdf/10.2116/analsci.16.477.pdf
- https://link.springer.com/article/10.2116/analsci.16.477/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/16/5/16_5_477/_pdf
- https://search.jamas.or.jp/link/ui/2001000576
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- 本文言語コード
- en
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- JaLC
- IRDB
- NDL
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