Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix

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  • OKADA Hiroki
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
  • KAJI Noritada
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
  • TOKESHI Manabu
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University
  • BABA Yoshinobu
    Department of Applied Chemistry, Graduate School of Engineering, Nagoya University MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University Plasma Nanotechnology Research Center, Nagoya University National Institute of Advanced Industrial Science and Technology (AIST) Institute for Molecular Science, National Institutes of Natural Sciences

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Abstract

Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model.

Journal

  • Analytical Sciences

    Analytical Sciences 24 (3), 321-325, 2008

    The Japan Society for Analytical Chemistry

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