Electrochemical Determination of Serotonin and the Competitive Adsorption with Dopamine at 5,5-Ditetradecyl-2-(2-trimethylammonioethyl)-1,3-dioxane Bromide Lipid Film Modified by Glassy Carbon Electrode

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A novel and effective approach to sensitively determine serotonin, known as 5-hydroxytryptamine (5-HT), has been proposed based on a 5,5-ditetradecyl-2-(2-trimethylammonioethyl)-1,3-dioxane bromide (DTDB) self-assembled lipid bilayer membrane modified glassy carbon electrode (DTDB/GCE). A DTDB/GCE shows the strong electrocatalysis for the oxidation of 5-HT, with the peak potential shifted to less positive value of 0.376 V vs. SCE, and effectively eliminates the interference from ascorbic acid (AA), even in the presence of 100-fold concentration of AA. Differential pulse voltammetry (DPV) gave a linear current for 5-HT from 2.0 × 10-7 to 1.0 × 10-5 M. At the DTDB/GCE, the oxidation of 5-HT was controlled by the adsorption process; for 5-HT coexisting with DA, the competitive adsorption was observed.

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  • Analytical Sciences

    Analytical Sciences 20 (6), 905-909, 2004

    社団法人 日本分析化学会

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