Time-Resolved Fluorometric Detection of DNA Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.

DOI PDF Web Site Web Site Web Site 2 Citations 48 References
  • YOSHIKAWA Kenji
    Department of Chemistry, Waseda University
  • YUAN Jingli
    Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
  • MATSUMOTO Kazuko
    Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)
  • KIMURA Hiroko
    Japan Science and Technology Corporation (JST) Department of Forensic Medicine, Juntendo University School of Medicine

Bibliographic Information

Other Title
  • Time-Resolved Fluorometric Detection of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label
  • Time-Resolved Fluorometric Detection of DNA Using a Tetradentate ベーターDiketonate Europium Chelate as a Label

Search this article

Abstract

A tetradentate β-diketonate europium chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chloro-sulfo-o-terphenyl(BHHCT)-Eu3+, was labeled to a streptavidin-bovine serum albumin conjugate for time-resolved fluorometric detection of λDNA by hybridization with a biotinylated probe DNA in a microtiter well. The method gave a detection limit of 23 pg/well (solid-phase measurement) and 4 pg/well (solution-phase measurement) for specific DNA.

Journal

  • Analytical Sciences

    Analytical Sciences 15 (2), 121-124, 1999

    The Japan Society for Analytical Chemistry

Citations (2)*help

See more

References(48)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top