A New Protein Conformation Indicator Based on Biarsenical Fluorescein with an Extended Benzoic Acid Moiety
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- NAKANISHI Jun
- Bioengineering Laboratory, RIKEN
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- MAEDA Mizuo
- Bioengineering Laboratory, RIKEN
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- UMEZAWA Yoshio
- Department of Chemistry, School of Science, The University of Tokyo
Bibliographic Information
- Other Title
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- New Protein Conformation Indicator Based on Biarsenical Fluorescein with an Extended Benzoic Acid Moiety
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Abstract
We demonstrate herein a new protein conformation indicator based on biarsenical fluorescein with an extended benzoic acid moiety. The present indicator is reactive to a genetically introduced tetracysteine motif (Cys-Cys-Xaa-Xaa-Cys-Cys, where Xaa is a noncysteine amino acid) of proteins. Compared to the original biarsenical fluorescein (FlAsH) and the biarsenical Nile red analogue (BArNile), the present indicator exhibited larger fluorescence intensity changes in response to Ca2+-induced conformational rearrangements of calmodulin. A calculation of the highest occupied molecular orbital (HOMO) level of the benzoic acid moiety of the indicator molecule supports possible involvement of a photoinduced electron transfer (PET) process. These results indicate that the present indicator is useful for sensitive detection of protein conformational changes.
Journal
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- Analytical Sciences
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Analytical Sciences 20 (2), 273-278, 2004
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679234607360
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- NII Article ID
- 10012046694
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD2cXivVClsLc%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 6843066
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- PubMed
- 15055950
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed