Direct Electrochemical Behavior of Cytochrome c on DNA-modified Glassy Carbon Electrode and Its Application to Cytochrome c Biosensor
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- LIU Yun-Chun
- School of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University
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- CUI Shi-Qiang
- School of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University
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- YANG Zhou-Sheng
- School of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University
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DNA was immobilized on glassy carbon electrodes to fabricate DNA-modified electrodes. The direct electron transfer of horse heart cytochrome c on DNA-modified glassy carbon electrode was achieved. A pair of well-defined redox peaks of cytochrome c appeared at Epc = -0.017 V and Epa = 0.009 V (vs. Ag/AgCl) in 10 mM phosphate buffer solution (pH 7.0) at a scan rate of 50 mV/s. The electron transfer coefficient (α) and the standard rate constant of the surface reaction (Ks) of cytochrome c on DNA-modified electrodes could be estimated to be 0.87 and 34.52 s-1, respectively. The DNA-modified glassy carbon electrode could be applied to detect cytochrome c by means of differential pulse voltammetry (DPV). The cathodic peak current was proportional to the quantity of cytochrome c in the range of 4.0 × 10-6 M to 1.2 × 10-5 M. The correlation coefficient is 0.996, and with the detection limit was 1.0 × 10-6 M (three times the ratio of signal to noise, S/N = 3).
収録刊行物
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- Analytical Sciences
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Analytical Sciences 22 (8), 1071-1074, 2006
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679234672768
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- NII論文ID
- 130004441317
- 10017650136
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 8011246
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- Web Site
- http://id.ndl.go.jp/bib/8011246
- https://ndlsearch.ndl.go.jp/books/R000000004-I8011246
- https://link.springer.com/content/pdf/10.2116/analsci.22.1071.pdf
- https://link.springer.com/article/10.2116/analsci.22.1071/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/22/8/22_8_1071/_pdf
- https://search.jamas.or.jp/link/ui/2006319712
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- 本文言語コード
- en
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